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serotonin transporter gene polymorphism is associatedwith delusions
in patients with AD.
Methods:
A total of 257 consecutive AD patients attending the
Alzheimer
’
s Evaluation Unit of the IRCCS
‘
Casa Sollievo della
Sofferenza
’
in San Giovanni Rotondo, Italy were included in this
study. Of these, 171 AD patients with delusions (AD-D) and 86 AD
patients without delusions (AD-noD).
All participants underwent a comprehensive evaluation with standar-
dized CGA,
Mini-Mental State Examination (MMSE),
and
Neuropsychiatric Inventory (NPI). Individuals were genotyped for
the 5HTTLPR polymorphism in blinded fashion.
Results:
No significant difference were showed between the two
groups on sex, mean age, educational level and scores in CGA. AD-D
patients showed significantly an higher cognitive impairment in
MMSE (p = 0.047), and an higher score in NPI (p < 0.0001) and NPI-
Distress (p < 0.0001) than AD-noD patients. Homozygosity for the L/L
genotype were associated with a lower MMSE (p = 0.011) and an
increased risk for delusions (p < 0.0001).
Conclusions:
This study showed that the 5HTTLPR polymorphism is
associated with delusions in AD, with important implications
regarding the mechanisms underlying this symptom. Because of
this, it could be possible to implement a personalized therapy for AD
patients with delusions.
P-080
A vitro study on mechanism of benefiting kidney herbs in treating
and preventing osteoarthritis
Jia Li, Aisong Zhu, Wanqing Xie, Hongxin Zheng.
Liaoning University of
Traditional Chinese Medicine
Objective:
To evaluate the effect of Icariin, Psoralen, Oleanolic and TSG
in regulating the expression of OA-related impacts BMP 2, BMP 4 and
Runx2. To detect the function of benefiting kidney herbs in improving
articular cartilage self-maintaining and approach the molecular
mechanism, by which means to demonstrate the relation between
kidney and articular cartilage in Chinese medicine theory, and more
comprehensively to extend the conception of
“
kidney governs bone
”
theory.
Method:
Mesenchymal cells were collected from i month old wildtype
micce and devided into blank control, control, Icariin, Psoralen,
Oleanolic acid and TSG groups. Cells were harvested for RNA extraction
on 48 h time points to detect the concentration of BMP2, BMP4,
and RUNX2 genes. Western-blotting was performed to examine the
protein expression of BMP2, BMP4, and RUNX2 on Day 5.
Result:
1. Comparing with control group, gene expression of BMP4 and
RUNX2 is significantly increaded in the other 4 herb groups (P < 0.05).
BMP2 gene expression is higher in Icariin group and TSG group
(P < 0.05). 2. Comparing with control group, protein expression of
BMP2 and BMP4 is dramatically increased. Runx2 protein expression is
stronge in Icariin group and TSG group.
Conclusion:
1. Icariin, Psoralen, Oleanolic acid and TSG, extracted from
benefiting kidney herbs, can up-regulate the gene and protein
expression of BMP2, BMP4 and RUNX2. 2. The melacuar mechanism
of benifiting kidney herbs in treating and preventing OA and slow
down the AC degradation may relate to the up-regulation of BMP2,
BMP4 and Runx2. 3.
“
Kidney governs bone
”
theory involves the its
regulation to articular cartilage.
Key words:
kidney, articular cartilage, Icariin, Psoralen, Oleanolic
acid, TSG
P-081
Assessment of health status by molecular measures inmiddle-aged
to old persons, ready for clinical use?
M.E.C. Waaijer
1
, R.G.J. Westendorp
2
, D. Goldeck
3
, D.A. Gunn
4
,
G. Pawelec
3
, M. Stijntjes
1,5
, P.E. Slagboom
5,6
, A.B. Maier
7,8
.
1
Department
of Gerontology and Geriatrics, Leiden University Medical Center, the
Netherlands;
2
Department of Public health and Center of Healthy Aging,
University of Copenhagen, Copenhagen, Denmark;
3
Department of
Internal Medicine II, Centre for Medical Research, University of Tübingen,
Germany;
4
Unilever Discover, Colworth Science Park, Sharnbrook,
Bedfordshire, UK;
5
Netherlands Consortium for Healthy Aging, Leiden
University Medical Center,
6
Department of Molecular Epidemiology,
Leiden University Medical Center,
7
Department of Human Movement
Sciences, MOVE Research Institute Amsterdam, VU University Amsterdam,
Amsterdam, The Netherlands;
8
Department of Medicine and Aged Care,
Royal Melbourne Hospital, University of Melbourne, Melbourne, Australia
Objectives:
In addition to measures already used in clinical practice,
molecular measures have been proposed to assess health status, but
these have not yet been introduced into clinical practice. We aimed to
test the association of functional capacity measures used in current
practice and molecular measures with age and health status.
Methods:
The cohort consisted of 178 middle-aged to old participants
of the Leiden Longevity Study (range 42
–
82 years). We tested
associations between functional capacity measures (physical tests:
grip strength, 4-meter walk, chair stand test; cognitive tests: Stroop
test, digit symbol substitution test and 15-picture learning test) with
age and with cardiovascular or metabolic disease as a measure of the
health status. These associations with age and health status were also
tested for molecular measures (C reactive protein (CRP), numbers of
senescent p16INK4a positive cells in the epidermis and dermis and
putative immunosenescence (presence of CD57+ T cells)).
Results:
All functional capacity measures were associated with age.
CRP and epidermal p16INK4a positivity were also associated with age,
but with smaller estimates. Grip strength and the Stroop test were
associated with cardiovascular or metabolic disease, as was epidermal
p16INK4a positivity. All associations with cardiovascular or metabolic
disease attenuated when adjusting for age.
Conclusion:
In conclusion, in middle-aged to old persons, the
molecular measures tested here were more weakly associated with
age and health status than functional capacity measures. Whether
these molecular measures associate more closely with health status in
the elderly or in specific groups of patients needs to be explored
further.
P-082
Altered mitochondrial quality control checkpoints in skeletal
muscle of older patients with cancer cachexia
E. Marzetti, M. Lorenzi, A. Picca, F. Tanganelli, F. Landi, R. Bernabei,
M. Bossola, R. Calvani.
Catholic University of the Sacred Hear
Introduction:
Cancer cachexia (CC) is a multifaceted debilitating
syndrome featured by body weight loss mainly due to skeletal muscle
wasting. The mitochondrial involvement in muscle wasting has
attained consensus over time, although its role in the pathogenesis
of CC is still unclear. We investigated mitochondrial quality control
(MQC) signalling in muscle and cachexia in patients with gastric
cancer.
Methods:
Biopsies from the rectus abdominis muscle of 18 older
patients with gastric cancer (9 with CC and 9 non-cachectic (NCC))
were collected and assayed for the expression of a set of MQC
mediators.
Results:
Mitochondrial plasticity was analyzed first, and no changes
were found between groups in the protein content of either mitofusin
2 (Mfn2) or optic atrophy protein 1 (OPA1). CC patients, instead,
showed an up-regulation of fission protein 1 (Fis1) gene expression
relative to NCC. The calculation of the
“
fusion index
”
(Mfn/Fis1 protein
ratio), as a measure of cell ability to compensate for mitochondrial
impairment, revealed a failure for such a compensation in CC patients.
As for mitophagy, there was no difference in the expression of
the PTEN-induced putative kinase 1 (PINK1) between groups but,
interestingly, the protein ratio of the lipidated and non-lipidated form
of microtubule-associated protein 1 light chain 3B (LC3B II/LC3B I), an
index of ongoing autophagy, showed a decrease in CC patients
compared with NCC counterpart. Neither the protein expression of
autophagy-associated protein 7 (Atg7) and lysosome-associated
membrane protein 2 (LAMP-2) nor the mRNA abundance of the
Poster presentations / European Geriatric Medicine 7S1 (2016) S29
–
S259
S49