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serotonin transporter gene polymorphism is associatedwith delusions

in patients with AD.


A total of 257 consecutive AD patients attending the


s Evaluation Unit of the IRCCS

Casa Sollievo della


in San Giovanni Rotondo, Italy were included in this

study. Of these, 171 AD patients with delusions (AD-D) and 86 AD

patients without delusions (AD-noD).

All participants underwent a comprehensive evaluation with standar-

dized CGA,

Mini-Mental State Examination (MMSE),


Neuropsychiatric Inventory (NPI). Individuals were genotyped for

the 5HTTLPR polymorphism in blinded fashion.


No significant difference were showed between the two

groups on sex, mean age, educational level and scores in CGA. AD-D

patients showed significantly an higher cognitive impairment in

MMSE (p = 0.047), and an higher score in NPI (p < 0.0001) and NPI-

Distress (p < 0.0001) than AD-noD patients. Homozygosity for the L/L

genotype were associated with a lower MMSE (p = 0.011) and an

increased risk for delusions (p < 0.0001).


This study showed that the 5HTTLPR polymorphism is

associated with delusions in AD, with important implications

regarding the mechanisms underlying this symptom. Because of

this, it could be possible to implement a personalized therapy for AD

patients with delusions.


A vitro study on mechanism of benefiting kidney herbs in treating

and preventing osteoarthritis

Jia Li, Aisong Zhu, Wanqing Xie, Hongxin Zheng.

Liaoning University of

Traditional Chinese Medicine


To evaluate the effect of Icariin, Psoralen, Oleanolic and TSG

in regulating the expression of OA-related impacts BMP 2, BMP 4 and

Runx2. To detect the function of benefiting kidney herbs in improving

articular cartilage self-maintaining and approach the molecular

mechanism, by which means to demonstrate the relation between

kidney and articular cartilage in Chinese medicine theory, and more

comprehensively to extend the conception of

kidney governs bone



Mesenchymal cells were collected from i month old wildtype

micce and devided into blank control, control, Icariin, Psoralen,

Oleanolic acid and TSG groups. Cells were harvested for RNA extraction

on 48 h time points to detect the concentration of BMP2, BMP4,

and RUNX2 genes. Western-blotting was performed to examine the

protein expression of BMP2, BMP4, and RUNX2 on Day 5.


1. Comparing with control group, gene expression of BMP4 and

RUNX2 is significantly increaded in the other 4 herb groups (P < 0.05).

BMP2 gene expression is higher in Icariin group and TSG group

(P < 0.05). 2. Comparing with control group, protein expression of

BMP2 and BMP4 is dramatically increased. Runx2 protein expression is

stronge in Icariin group and TSG group.


1. Icariin, Psoralen, Oleanolic acid and TSG, extracted from

benefiting kidney herbs, can up-regulate the gene and protein

expression of BMP2, BMP4 and RUNX2. 2. The melacuar mechanism

of benifiting kidney herbs in treating and preventing OA and slow

down the AC degradation may relate to the up-regulation of BMP2,

BMP4 and Runx2. 3.

Kidney governs bone

theory involves the its

regulation to articular cartilage.

Key words:

kidney, articular cartilage, Icariin, Psoralen, Oleanolic

acid, TSG


Assessment of health status by molecular measures inmiddle-aged

to old persons, ready for clinical use?

M.E.C. Waaijer


, R.G.J. Westendorp


, D. Goldeck


, D.A. Gunn



G. Pawelec


, M. Stijntjes


, P.E. Slagboom


, A.B. Maier





of Gerontology and Geriatrics, Leiden University Medical Center, the



Department of Public health and Center of Healthy Aging,

University of Copenhagen, Copenhagen, Denmark;


Department of

Internal Medicine II, Centre for Medical Research, University of Tübingen,



Unilever Discover, Colworth Science Park, Sharnbrook,

Bedfordshire, UK;


Netherlands Consortium for Healthy Aging, Leiden

University Medical Center,


Department of Molecular Epidemiology,

Leiden University Medical Center,


Department of Human Movement

Sciences, MOVE Research Institute Amsterdam, VU University Amsterdam,

Amsterdam, The Netherlands;


Department of Medicine and Aged Care,

Royal Melbourne Hospital, University of Melbourne, Melbourne, Australia


In addition to measures already used in clinical practice,

molecular measures have been proposed to assess health status, but

these have not yet been introduced into clinical practice. We aimed to

test the association of functional capacity measures used in current

practice and molecular measures with age and health status.


The cohort consisted of 178 middle-aged to old participants

of the Leiden Longevity Study (range 42

82 years). We tested

associations between functional capacity measures (physical tests:

grip strength, 4-meter walk, chair stand test; cognitive tests: Stroop

test, digit symbol substitution test and 15-picture learning test) with

age and with cardiovascular or metabolic disease as a measure of the

health status. These associations with age and health status were also

tested for molecular measures (C reactive protein (CRP), numbers of

senescent p16INK4a positive cells in the epidermis and dermis and

putative immunosenescence (presence of CD57+ T cells)).


All functional capacity measures were associated with age.

CRP and epidermal p16INK4a positivity were also associated with age,

but with smaller estimates. Grip strength and the Stroop test were

associated with cardiovascular or metabolic disease, as was epidermal

p16INK4a positivity. All associations with cardiovascular or metabolic

disease attenuated when adjusting for age.


In conclusion, in middle-aged to old persons, the

molecular measures tested here were more weakly associated with

age and health status than functional capacity measures. Whether

these molecular measures associate more closely with health status in

the elderly or in specific groups of patients needs to be explored



Altered mitochondrial quality control checkpoints in skeletal

muscle of older patients with cancer cachexia

E. Marzetti, M. Lorenzi, A. Picca, F. Tanganelli, F. Landi, R. Bernabei,

M. Bossola, R. Calvani.

Catholic University of the Sacred Hear


Cancer cachexia (CC) is a multifaceted debilitating

syndrome featured by body weight loss mainly due to skeletal muscle

wasting. The mitochondrial involvement in muscle wasting has

attained consensus over time, although its role in the pathogenesis

of CC is still unclear. We investigated mitochondrial quality control

(MQC) signalling in muscle and cachexia in patients with gastric



Biopsies from the rectus abdominis muscle of 18 older

patients with gastric cancer (9 with CC and 9 non-cachectic (NCC))

were collected and assayed for the expression of a set of MQC



Mitochondrial plasticity was analyzed first, and no changes

were found between groups in the protein content of either mitofusin

2 (Mfn2) or optic atrophy protein 1 (OPA1). CC patients, instead,

showed an up-regulation of fission protein 1 (Fis1) gene expression

relative to NCC. The calculation of the

fusion index

(Mfn/Fis1 protein

ratio), as a measure of cell ability to compensate for mitochondrial

impairment, revealed a failure for such a compensation in CC patients.

As for mitophagy, there was no difference in the expression of

the PTEN-induced putative kinase 1 (PINK1) between groups but,

interestingly, the protein ratio of the lipidated and non-lipidated form

of microtubule-associated protein 1 light chain 3B (LC3B II/LC3B I), an

index of ongoing autophagy, showed a decrease in CC patients

compared with NCC counterpart. Neither the protein expression of

autophagy-associated protein 7 (Atg7) and lysosome-associated

membrane protein 2 (LAMP-2) nor the mRNA abundance of the

Poster presentations / European Geriatric Medicine 7S1 (2016) S29